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2 Activities carried out and results
2.1 Testing new products and methods
Of the three representative IGRs selected, triflumuron (Alsystin, Bayer Co., Leverkusen) was tested most intensively, since this product was effective at lower concentrations than teflubenzuron (Nomolt, Shell Corp.) and diflubenzuron (Dimilin, Duphar) (about 1/3 lower amounts of active ingredients required). As botanicals, we selected neem oil from India (SROM Co.) (largely standardised; readily available in amounts of up to 10,000 litres and more, while being relatively economical at <6 US $/l), as well as M. volkensii powder with constant content of active ingredient. The experiments with Metarhizium flavoviride spores against hopper bands were performed in collaboration with the IITA, DFPV.
2.1.1 Effectiveness tests on insect growth regulators (IGRs), botanicals and fungal spores
The IGRs and botanicals used gave dose-dependent mortality rates of 80 - 100 % after 6 - 12 days. Fairly comparable mortality rates were also achieved with the fungal spores, but in this case more than I2 days were required for effectiveness. The most important mortality rates obtained for larvae and adults during the period of the project are summarised in Tables 1 and 2.
Tab. 1 Mortality rates (in %) of S. gregaria adults (field cage trials)
|
Products |
Class |
Dosage |
Highest mortality (in %) |
|||
|
1991/1992 after |
1992/1993 after |
|||||
|
6 days |
12 days |
6 days |
12 days |
|||
|
Neem oil |
Botanicals |
1 g a.i./ha |
88 |
92 |
38 |
95 |
|
Azal-F |
1 g a.i./ha |
62 |
80 |
- |
- |
|
|
Melia volkensii |
10 g a.i./ha |
- |
- |
32 |
88 |
|
|
Metarhizium flavoviride |
Mycocides |
1013 spores/ha |
0 |
88 |
- |
95 |
|
Beauveria |
1013 spores/ha |
20 |
50 |
- |
- |
|
|
bassiana |
2.5x1013 spores/ha |
- |
- |
33 |
88 |
|
|
Triflumuron |
Benzoyl phenyl |
25 g a.i./ha |
- |
- |
25 |
70 |
|
Teflubenzuron |
ureas (BPU) |
50 g a.i./ha |
48 (1990) |
69 (1990) |
30 |
65 |
Tab. 2 Mortality rate (in %) of S. gregaria larvae
|
Highest mortality in % |
||||||||||
|
1991/92 after |
1992/93 after |
1993/1994 after |
1994/95 after |
|||||||
|
Products |
Class |
Dosage |
6 days |
12 days |
6 days |
12 days |
6 days |
10 days |
6 days |
12 days |
|
Neem oil |
Botanicals |
0.8 - 2.4 g a.i./ha |
38 |
90 |
33 |
60 |
80 |
85 |
38 |
56 |
|
Neem Azal |
1 g a.i./ha |
50 |
94 |
- |
- |
45 |
78 |
- |
- |
|
|
Melia volkensii |
10 g a.i./ha |
- |
- |
10 |
501 |
42 |
66 |
46 |
91 |
|
|
Nosema locustae |
Microbial pesticides |
baits |
0 (1990) |
0 (1990) |
- |
- |
- |
- |
- |
- |
|
Beauveria bassiana |
2.5x1013 |
- |
- |
42 |
66 |
- |
- |
- |
- |
|
|
Metarhizium flavoviride |
- |
- |
- |
- |
50 |
92 |
47 |
98 |
||
|
Triflumuron |
Benzoyl phenyl ureas |
12.5 g a.i./ha |
- |
- |
48 |
72 |
- |
- |
- |
- |
|
25 g a.i./ha |
70 |
100 |
432 |
702 |
403 |
60 - 703 |
755 |
1005 |
||
|
50 g a.i./ha |
- |
- |
60 |
100 |
- |
804 |
406 |
936 |
||
|
Teflubenzuron |
25 g a.i./ha |
40 |
81 |
- |
- |
- |
- |
- |
- |
|
|
50 g a.i./ha |
- |
- |
72 |
82 |
- |
- |
- |
- |
||
|
Diflubenzuron |
60 g a.i./ha |
- |
- |
- |
- |
- |
- |
44 |
73 |
|
In all tests, the mortality rate for untreated controls was <10 %, and for the controls treated with solvent alone <22 %. Source of the products: Neem oils (Prof. Schmutterer, Univ. Giessen; Trifolio Co., Lahnau; SROM Corp., India); M. volkensii extracts (Prof. Rembold, MPI Munich; Prof. Mwangi, Univ. Nairobi); benzoyl phenyl ureas (Bayer Corp., Leverkusen; Shell, Inc., Ingelheim; and Rhône-Poulenc Co.); mycocides (Dr. Prior, IIBC; Dr. Crawford, Myco Tech Co.; Dr. Welling, BBA).
1. M. volkensii treated larvae did not undergo adult moulting
2 Topical application infield-cage trials
3 Topical application on a hopper band
4 Barrier application
5 Topical application on sleeping places
6 Barrier treatment